Study of trisomy 22 and inversion 16 in acute myeloid leukemia.
- Author:
Hui-fen ZHOU
1
;
Jian-yong LI
;
Jin-lan PAN
;
Hai-rong QIU
;
Li-juan CHEN
;
Jie-ying HU
;
Yun-feng SHEN
;
Wei XU
;
Yong-quan XUE
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Chromosome Inversion; Chromosomes, Human, Pair 16; genetics; Chromosomes, Human, Pair 22; genetics; Female; Humans; In Situ Hybridization, Fluorescence; Leukemia, Myeloid, Acute; genetics; Male; Middle Aged; Trisomy
- From: Chinese Journal of Hematology 2007;28(1):11-14
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the value of trisomy 22 ( +22) in the diagnosis of inv(16) acute myeloid leukemia (AML).
METHODSInterphase fluorescence in situ hybridization (FISH) was performed in 18 AML patients with +22. The probe was two-color break apart probe for CBFbeta with SpectrumRed on the centromeric side and SpectrumGreen on the telomeric side. The FISH results were compared with that of R-banding conventional cytogenetics (CC). Multiplex FISH (M-FISH) was used to analyze the relationship of +22 and inv(16).
RESULTSCC revealed inv(16) in none of the 18 AML, with +22, but FISH revealed inv (16) in 11 of them and del( 16) (q22) in one. As CC results, 9 of the 11 cases were sole +22, one complicated with trisomy 8, and one del(16) (q22). Four patients with +22 and inv(16) were analyzed by M-FISH and revealed +22 only.
CONCLUSION+22 can be regarded as an important marker for the diagnosis of inv(16) AML.
