Identification of heplipin inducing apoptosis-related genes in KG-1 human leukemia cells by differential display RT-PCR.
- Author:
Li LI
1
;
Zhen-lan SONG
;
Dong-gang XU
;
Jia-xi WANG
Author Information
- Publication Type:Journal Article
- MeSH: ATPases Associated with Diverse Cellular Activities; Apoptosis; drug effects; genetics; Cell Line, Tumor; Fatty Acids, Unsaturated; pharmacology; Gene Expression Profiling; Humans; Leukemia; genetics; pathology; Proteasome Endopeptidase Complex; genetics; RNA, Messenger; genetics; Wnt Proteins; genetics
- From: Chinese Journal of Hematology 2007;28(1):22-26
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether Heplipin can induce KG-1 cell apoptosis and explore apoptosis related differentially expressed genes in KG-1 leukemia cell before and after Heplipin induction.
METHODSDNA distribution and DNA electrophoresis were used to prove that Heplipin can induce KG-1 cell apoptosis. The differential display reverse transcription-polymerase chain reaction (DDRT-PCR) was adopted to screen differentially expressed genes before and after Heplipin induction of KG-1 cells for 16 hours and 20 hours. The differentially expressed genes were cloned and analyzed.
RESULTSHeplipin could induce KG-1 cell apoptosis. There were differentially expressed genes in KG-1 cells before and after induction. Wnt13 and ATPase 3 were apoptosis related differentially downregulated genes after Heplipin induction. Conclusion Heplipin can induce KG-1 cell apoptosis. Heplipin induced KG-1 cell apoptosis is related with Wntl3 and ATPase3 (PSMC3). It is the first report that Wnt13 was detected in leukemia cell line.
