Expression of AML1/ETO9a isoform in acute myeloid leukemia-M2 subtype.
- Author:
Yu-qing MIAO
1
;
Zi-xing CHEN
;
Jun HE
;
Jian-nong CEN
;
Xiao-jing BAO
;
Qiao-cheng QIU
;
Dong-er ZHANG
;
Ming YAN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Core Binding Factor Alpha 2 Subunit; genetics; metabolism; Female; Gene Expression; Humans; Karyotyping; Leukemia, Myeloid, Acute; genetics; metabolism; Male; Middle Aged; Oncogene Proteins, Fusion; genetics; metabolism; Protein Isoforms; genetics; metabolism; RUNX1 Translocation Partner 1 Protein
- From: Chinese Journal of Hematology 2007;28(1):27-29
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of AML1/ETO9a isoform in the acute myeloid leukemia (AML)-M2 patients.
METHODSExpressions of AML1/ETO fusion gene and AML1/ETO9a isoform were detected by using reverse transcriptase-polymerase chain reaction (RT-PCR) in leukemia patients, MDS patients, leukemia cell lines and healthy subjects. Karyotype was studied by R-banding technique.
RESULTIn 30 newly diagnosed AML-M2 patients 15 were found to express AML1/ETO9a isoform, while the rest including 20 AML-M2CR, 18 other subtypes of AML, 5 chronic myelogenous leukemia (CML), 3 myelodysplastic syndromes (MDS), 3 leukemia cell lines (NB4, KG-1, K562) and 5 healthy subjects were AML1/ETO9a negative. Among the 15 AML/ETO9a isoform expressing cases, 13 were demonstrated t(8;21) translocation and AML1/ETO expression.
CONCLUSIONIsoform AML1/ETO9a was correlated to AML/M2, and it may promote the development of leukemia in combination with the AML1/ETO fusion gene.
