Effect of arsenic trioxide on VEGF/R and MMP-2, 9 expressed in K562 cells.
- Author:
Yu ZHANG
1
;
Jian-Dong ZHANG
;
Jian GU
;
Li MA
;
Wei-Gan SHEN
;
Xiao-Ling WANG
;
Hong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Arsenicals; pharmacology; Cell Proliferation; drug effects; Humans; K562 Cells; Matrix Metalloproteinase 2; metabolism; Matrix Metalloproteinase 9; metabolism; Oxides; pharmacology; Receptors, Vascular Endothelial Growth Factor; metabolism; Vascular Endothelial Growth Factor A; metabolism
- From: Chinese Journal of Hematology 2007;28(2):107-110
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of arsenic trioxide (As2 O3) on the level of VEGF, VEGFR and the activity of MMP-2, 9 in K562 cells.
METHODSThe inhibition ratio of K562 cell was detected by MTT assay, the level of VEGF by Enzyme-linked immunosorbent assay (ELISA), the expression ratio of VEGFR by flow cytometry (FCM), and the activity of MMP-2, 9 by gelatin zymography assay.
RESULTS(1) The IC50 of K562 cells was (2.12 +/- 0.11) micromol/L. Proliferation of K562 cells was significantly inhibited at the concentration of 0.4 - 6.4 micromol/L As2 O3 (P < 0.05). (2) The expression of VEGF was slightly up-regulated by 0.05 micromol/L As2 O3 (P > 0.05) and prominently inhibited by 0.4 micromol/L and 3.2 micromol/L As2 O3 (P < 0.05). As2 O3 had no influence on VEGFR. (3) The activity of MMP-2 and 9 was partly inhibited by 0.05 micromol/L As2 O3 incubated 72 hours and by 0.4, 3.2 micromol/L, As2 O3. With the increase of As2 O3 concentration and the incubation time, the inhibited effect on MMP-2 and 9 was enhanced.
CONCLUSIONSAs2 O3 may down-regulate the expression of VEGF and inhibit the activity of MMP-2 and 9.
