Study on molecular cytogenetic abnormalities in multiple myeloma.
- Author:
Shu-Yan LIU
1
;
Jian-Yong LI
;
Li-Juan CHEN
;
Jin-Wen HUANG
;
Jin-Lan PAN
;
Hai-Rong QIU
;
Yun-Feng SHEN
;
Wei XU
;
Yong-Quan XUE
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Chromosome Aberrations; Female; Gene Deletion; Gene Rearrangement; Humans; Immunoglobulin Heavy Chains; genetics; In Situ Hybridization, Fluorescence; Male; Middle Aged; Multiple Myeloma; genetics; Plasma Cells
- From: Chinese Journal of Hematology 2007;28(4):223-226
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the molecular cytogenetic abnormalities in multiple myeloma (MM).
METHODSBone marrow plasma cells from 23 previously untreated MM patients were purified by CD138 McAb magnetic cell sorting system, and a panel of probes for interphase fluorescence in situ hybridization were used to detect the 13q14 deletion, p53 deletion and IgH gene translocation in the sorted MM cells.
RESULTSAmong 23 MM patients, 13q14 deletion was observed in 10 (43.5%) cases, with the positive rate of 13q14 deleted cells ranged from 79% to 96%; 14q32 translocation was observed in 11 (47.8%) cases; 13q14 deletion and 14q32 translocation were simultaneously observed in 7 (30.4%) cases; and p53 deletion was observed in none of the 23 cases.
CONCLUSIONThe frequency of 13q14 deletion and IgH gene translocation in multiple myeloma are high; and the relationship between 13q14 deletion, IgH gene translocation and prognosis is worth further investigating.
