Effect of Taohong Qinlian Decoction on HMGB1 in Septic Rat Cardiac Muscle.
- Author:
Zhong-min GU
;
Shao-bin LIN
;
Cai-jun LIU
;
Xin-xin QI
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; pharmacology; HMGB1 Protein; metabolism; Heart; drug effects; Interleukin-6; metabolism; Male; Myocardium; metabolism; pathology; Random Allocation; Rats; Rats, Wistar; Sepsis; pathology; Troponin I; metabolism; Tumor Necrosis Factor-alpha; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2016;36(4):445-448
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the levels of high mobility group box-1 protein (HMGB1), tumor necrosis factor-alpha (TNF-α), IL-6, troponin I (Tn I) release in septic rats, and to explore themechanism of Taohong Qinlian Decoction (TQD) in the treatment of septic myocardial injury.
METHODSA total of 48 healthy male Wistar rats of clean grade were randomly divided into the sham-operation group (Sham), the sepsis model group (CLP), and the TQD treatment group (ZY), 16 in each group. Concen-trations of TNF-α, IL-6, Tn I, and HMGB1 expression were detected in each group at 24 and 48 h after operation. Pathological changes of cardiac muscle were observed under light microscope.
RESULTSConcentrations of TNF-α, IL-6, Tn I and HMGB1 at 24 and 48 h after operation were significantly higher in the CLP group than in the Sham group (P < 0.01). Concentrations of TNF-α, IL-6, Tn I, and HMGB1 at 24 and 48 h after operation were significantly lower in the ZY group than in the CLP group (P < 0.05). Myocardial injury occurred in the CLP and the ZY group under light microscope. And this injury was more severe in the CLP group than in the ZY group.
CONCLUSIONTQL could reduce the level of sepsis-related inflammatory cytokines and protect myocardium in septic rats.