Content detection of bacterial endotoxin in two kinds of injection by gelatin technique.
- Author:
Shuangrong GAO
1
;
Ting LIU
;
Jing GUO
;
Yan YI
;
Ran HAO
;
Chunying LI
;
Chunyu CAO
;
Yong ZHAO
;
Lianqiang HUI
;
Aihua LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drug Contamination; Drugs, Chinese Herbal; analysis; Endotoxins; analysis; Horseshoe Crabs; Limulus Test; methods
- From: China Journal of Chinese Materia Medica 2010;35(11):1405-1409
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect content of bacterial endotoxin in Yuxingcao and Qingkailing injections by specific and nonspecific tachypleus amebocyte lysate technique for in order to investigate the feasibility of specific tachypleus amebocyte lysate technique for detecting bacterial endotoxin in traditional Chinese drug injections.
METHODDifferent batches of Yuxingcao and Qingkailing injections were detected by specific and nonspecific tachypleus amebocyte lysate kits.
RESULTYuxingcao injection could be detected by specific and nonspecific tachypleus amebocyte lysate technique, Whereas Qingkailing injection could be detected only by specific tachypleus amebocyte lysate.
CONCLUSIONUsing specific tachypleus amebocyte lysate as a substitute for nonspecific tachypleus amebocyte lysate is an effective method for detecting content of bacterial endotoxin in Qingkailing injection.