Effect of polysaccharides in processed Sibiraea on immunologic function of immunosuppression mice.
- Author:
Bowen DUAN
1
;
Yun LI
;
Xin LIU
;
Yongjian YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Female; Humans; Immunity; drug effects; Immunocompromised Host; drug effects; Male; Mice; Organ Size; drug effects; Polysaccharides; administration & dosage; Random Allocation; Rosaceae; chemistry; Spleen; drug effects; immunology
- From: China Journal of Chinese Materia Medica 2010;35(11):1466-1469
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of polysaccharides in processed Sibiraea on the immunologic function of immunosuppression mice.
METHODThe immunosuppressed mice were induced by cyclophosphamide. After the treatment, the organ weight index and the delayed type hypersensitivity of the mice were investigated. The humoral immune function was determined by serum hemolysin assay. Non-specific immune function was determined by carbon clearance method. Cellular immune function was determined by spleen lymphocyte proliferation test. Two hundred kunming mice were randomly divided into five groups: normal controls, model group, low-dose group (110 mg x kg(-1)), middle-dose group (220 mg x kg(-1)), high-dose group (440 mg x kg(-1)). Drugs were given to the mice by oral gavage every day.
RESULTThe immunosuppressed mice treated with Sibiraea polysibcharide at intragastrica dose of 110-440 mg x kg(-1) have increased weight of the immune organs, increased content of DTH and content in serum hemolysin lgG and lgM. Mean while the rate of carbon clearance was enhanced and the proliferation of spleen lymphocyte was increased.
CONCLUSIONPolysaccharides in processed Sibiraea can increase the weight of the immune organs. At the same time, non-specific immune, DTH, humoral immune and cellular immune function were enhanced significantly.
