Protective effects of asiaticoside on sepsis-induced acute kidney injury in mice.
- Author:
Jiajia ZHENG
1
;
Li'na ZHANG
;
Mengjiao WU
;
Xiaohui LI
;
Li ZHANG
;
Jingyuan WAN
Author Information
- Publication Type:Journal Article
- MeSH: Acute Kidney Injury; drug therapy; etiology; prevention & control; Animals; Disease Models, Animal; Female; Humans; Male; Mice; Plant Extracts; administration & dosage; Random Allocation; Sepsis; complications; Triterpenes; administration & dosage
- From: China Journal of Chinese Materia Medica 2010;35(11):1482-1485
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe present study is to investigate the protective effects of asiaticoside on sepsis-induced acute kidney injury in mice.
METHODWith the sepsis induced by cecal ligation and puncture (CLP), forty eight kunming mice were randomly divided into four groups as sham operated, CLP treated, CLP + asiaticoside 15, 45 mg x kg(-1) groups. General conditions and the amount of dead rate of mice were observed. The BUN and Cr levels were observed by the kits. IL-6 in serum was assayed by enzyme-linked immunosorbent assay (ELISA). Kidney tissues were harvested for determination of iNOS expression by Western blotting analysis. The pathologic changes were observed under electron microscope via hematoxylin-eosin (HE) stain.
RESULTCompared with CLP group, the death rate, the levels of BUN, Cr, IL-6, and iNOS protein expression of asiaticoside groups were significantly reduced. The pathologic changes in kidney tissues induced by sepsis were significantly attenuated dose-dependently by asiaticoside under electron microscope.
CONCLUSIONAsiaticoside has protective effects against sepsis-induced acute kidney injury, which were probably associated with the inhibition of IL-6 in serum and iNOS protein in kidney tissues.
