Isolation and identification of endophytic fungi from different swollen root of Rehmannia glutinosa.
- Author:
Jing LI
1
;
Min WANG
;
Zhongping LIN
;
Qingjun YUAN
;
Rongmin YU
;
Luqi HUANG
Author Information
1. College of Pharmacy, Jinan University, Guangzhou 510632, China.
- Publication Type:Journal Article
- MeSH:
DNA, Fungal;
genetics;
DNA, Ribosomal;
genetics;
Fungi;
classification;
genetics;
isolation & purification;
Molecular Sequence Data;
Plant Roots;
microbiology;
Rehmannia;
microbiology
- From:
China Journal of Chinese Materia Medica
2010;35(13):1679-1683
- CountryChina
- Language:Chinese
-
Abstract:
The swollen root of Rehmannia glutinosa is used as one kind of important Chinese traditional medicine. The root of R. glutinosa usually swelled in rotational cropping but not in continuous cropping. The rhizosphere microorganisms of R. glutinosa under different farming condition were thought related to that. In this study, the endophytic fungi in the root of R. glutinosa growing in various soil conditions were isolated for the study of the relationship between the microorganisms and the root enlargement of their host plants. The dominant endophytes, Verticillium spp., Fusarium oxysporum, F. redolens and Ceratobasidium spp. were identified by morphological observation and 18S rDNA and ITS sequence analysis. The preliminary investigation showed that the excessive growth of Verticillium and Fusarium genus fungi is unfavorable for the R. glutinosa root swelling, but Ceratobasidium fungi has no effects on the root enlargement.
