Determination of tanshinol and protocatechuic aldehyde in shenkangling granula by HPLC.
- Author:
Kedan CHU
1
;
Jian ZHENG
;
Wei XU
;
Weirong XIE
;
Xiaoqin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Benzaldehydes; analysis; Catechols; analysis; Chromatography, High Pressure Liquid; methods; Dosage Forms; Drugs, Chinese Herbal; analysis
- From: China Journal of Chinese Materia Medica 2010;35(13):1699-1701
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for gradient elution determination of danshensu and protocatechuic aldehyde in Shengkangling granula.
METHODThe analysis was carried on a column of Hypersil ODS2-C18 with a mobile phase consisted of methanol-1% glacial acetic acid 0-8 min (5:95), 8-11 min (6:94-5:95), 11-20 min (5:95) gradient elution at the flow rate of 1 mL x min(-1). The detection wavelength was 280 nm.
RESULTThe linearity was obtained in the range of 0.0504-0.504 microg (r = 0.9998) for tanshinol and 0.1090-1.090 microg (r = 0.9999) for protocatechuic aldehyde, respectively. The average recoveries of tanshinol and protocatechuic aldehyde in Shenkangling granula were 98.40% and 98.85%, and the RSDs were all less than 2.0%.
CONCLUSIONThe method is simple, accurate and rapid. It may be suitable for the usual quality control of tanshinol and protocatechuic aldehyde in Shengkangling granula.
