Simultaneous determination of chlorogenic acid, cryptochlorogenic acid, caffeic acid, naringin, hesperidin and linarin in xiao'erjinning oral liquid by an HPLC method.
- Author:
Jing WANG
1
;
Xinzhuang ZHANG
;
Xiaoyan WU
;
Xiaoli ZHAO
;
Liuqing DI
;
Qing GUO
;
Shouchuan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Caffeic Acids; analysis; Chlorogenic Acid; analysis; Chromatography, High Pressure Liquid; methods; Dosage Forms; Drugs, Chinese Herbal; analysis; Flavanones; analysis; Glycosides; analysis; Hesperidin; analysis
- From: China Journal of Chinese Materia Medica 2010;35(13):1702-1705
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a HPLC method for the simultaneous determination of chlorogenic acid, cryptochlorogenic acid, caffeic acid, naringin, hesperidin and linarin in xiao' erjinning oral liquid.
METHODThe chromatographic separation was achieved on a Lichrospher C18 (4.6 mm x 250 mm, 5 microm) column with a mobile phase which was composed of acetonitrile(A) and 0.4% phosphoric acid(B) for gradient elution (10:90-18:82-27:73). The flow rate was (0.8-1.1-0.8) mL x min(-1), the column temperature was 30 degrees C and the detection wavelength was set at 300 nm.
RESULTThe results showed that 6 effective components were separated well and showed good linearity. The average recoveries were between 95%-105%.
CONCLUSIONThe method is proved to be rapid, accurate, credible and repeatable. It can be used for the quality control of Xiao'erjinning oral liquid.
