Simultaneous determination of five lignans in Schisandra chinensis by HPLC.
- Author:
Zuo DING
1
;
Weiwei LU
;
Hongbo LI
;
Junfan FU
;
Zhongyun PIAO
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Lignans; analysis; isolation & purification; Plant Extracts; analysis; isolation & purification; Schisandra; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(13):1728-1730
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a HPLC quantitative method for determination of schisandrin, schisandrol B, schisantherin A, deoxyschisandrin and gamma-schisandrin in Fructus Schisandrae.
METHODA symmetry C18 column (4.6 mm x 250 mm, 5 microm) was used with methanol (A) and water (B) as mobile phases, in gradient elution. The gradient program was as follows: 0-36 min, changed from 60% A to 66% A, 36-65 min, to 80% A, 65-70 min, kept for 80% A, 70-75 min, to 100% A. The flow rate was 0.5 mL x min(-1) and detection wavelength was set at 254 nm.
RESULTThe linearities of schisandrin, schisandrol B, schisantherin A, deoxyschisandrin and gamma-schisandrin were in the ranges of 0.0214. 160 (r = 0.9999), 0.020-4.000 (r = 0.9999), 0.021-4.240 (r = 0.9999), 0.020-3.960 (r = 0.9999) and 0.021-4.200 (r = 0.9999). The average recoveries were 104.8%, 104.2%, 102.7%, 104.6%, 104.5%, respectively.
CONCLUSIONThe method developed in this study was reliable, and can be used for the quality control of the fruits of S. chinensis.
