Determination of prim-O-glucosylcimifugin and 5-O-methylvisammisoide in Saposhnikovia divaricata and HPLC fingerprint analysis.
- Author:
Lijie MA
1
;
Bin YANG
;
Xuefeng FENG
;
Xiaojie YIN
;
Hua LI
;
Xiaoguang GE
;
Lige ZHU
;
Jun CAO
Author Information
- Publication Type:Journal Article
- MeSH: Apiaceae; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; isolation & purification; Monosaccharides; analysis; isolation & purification; Quality Control; Xanthenes; analysis; isolation & purification
- From: China Journal of Chinese Materia Medica 2010;35(13):1731-1734
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop HPLC methods for the determination of prim-O-glucosylcimifugin and 5-O-methylvisammisoide in Saposhnicovia divaricata and of HPLC fingerprint to compare the wild and culture varieties.
METHODConditions of determination: Shimadzu C18 column (4.6 mm x 150 mm, 5 microm), methanol-water (40:60) as mobile phase with the flow rate of 1 mL x min(-1). The detection wavelength was 254 nm. Conditions of HPLC fingerprint: MG II C18 column (4.6 mm x 250 mm, 5 microm), the mobile phase was acetonitrile-water with the flow rate of 1 mL x min(-1), using linear gradient elution, the column temperature was 30 degrees C.
RESULTThe average recovery of prim-O-glucosylcimifugin was 99.6% (RSD 0.72%, n=6). The average recovery of 5-O-methylvisammisoide was 102.6% (RSD 0.88%, n=6). The contents of prim-o-glucosylcimifugin in wild and culture varieties were (4.96 +/- 2.59) and (3.61 +/- 1.82) mg x g(-1) respectively. The contents of 5-O-methylvisammisoide were (3.91 +/- 2.09) and (4.37 +/- 2.02) mg x g(-1) respectively. The compositions of S. divaricata were effective separated under the conditions of HPLC fingerprint.
CONCLUSIONThe HPLC determination method of prim-O-glucosylcimifugin and 5-O-methylvisammisoide is convenient and accurate. The HPLC fingerprint analysis method could be a basis for quality control and classification evaluate of S. divaricata.
