Extraction, isolation and purification for ginkgolide B.
- Author:
Chenfeng ZHANG
1
;
Minghui LI
;
Yun TANG
;
Yanhai ZHANG
;
Min SHI
;
Longsheng SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Chemical Fractionation; methods; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; isolation & purification; Ginkgo biloba; chemistry; Ginkgolides; analysis; isolation & purification; Lactones; analysis; isolation & purification; Plant Leaves; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(15):1961-1964
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a simple extraction, isolation and purification method for ginkgolide B from ginkgo leaf.
METHODThe optimum conditions of extraction, isolation and purification were studied by taking the transfer rate of ginkgolide B as index.
RESULTGinkgo leaf was extracted with 70% ethanol for three times, the extracts were concentrated to remove ethanol and diluted by water till the crude drug density reached 0.1 g x mL(-1). The dilution was adsorbed with HPD-450 macroporous resin. The impurities were eluted with 20% ethanol and ginkgolide B was eluted with 80% ethanol. Then the 80% ethanol eluant was concentrated and crystallized. Finally the crude crystals were recrystallized with isopropanol. The purity of the ginkgolide B recrystallization was 95%.
CONCLUSIONThe process was stable and easy to operate, which was suited to industrialized production.
