Determination of methyl nonyl ketone in volatile oil from herbs of Houttuynia cordata by GC-MS.
- Author:
Lixin YANG
1
;
Yongxin ZHANG
;
Hong YI
;
Hua YANG
;
Qiwei ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Gas Chromatography-Mass Spectrometry; methods; Houttuynia; chemistry; Ketones; analysis; Oils, Volatile; analysis; Plant Extracts; analysis
- From: China Journal of Chinese Materia Medica 2010;35(15):1987-1989
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a GC-MS method for the determination of methyl nonyl ketone in the volatile oil from the herbs of Houttuynia cordata.
METHODThe sample was split in the 240 degrees C injection port, with 20:1 split ratio, and separated on a DB-5 (30 m x 0.25 mm, 0.25 microm film thickness) fused silica column with helium as the carrier gas. The temperature program was as follows: 100 degrees C for 2 min, the 5 degrees C x min(-1) to 150 degrees C, then 15 degrees C x min(-1) to 200 degrees C, and kept for 10 min. The MS transfer line temperature was set to 250 degrees C, the MS source temperature was set to 200 degrees C. The ionization mode was electron ionization (EI) and the selective ion monitor mode was used.
RESULTA good linear relationship was constructed over the injection amount range of 5.5-110 ng of methyl nonyl ketone. The average recovery was 98.9%, and RSD was 2. 2%.
CONCLUSIONThe developed method was sensitive, accurate, and can be used for the determination of methyl nonyl ketone in the volatile oil and for the quality control of H. cordata.
