Determination of biological activity of extract from hirudo by N-benzoyl-L-arginine ethyl ester.
- Author:
Shixia GUAN
1
;
Can PENG
;
Haigang LI
;
Shuqin XU
;
Zhongwen YUAN
Author Information
1. College of Chinese Pharmacy, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. drguan@vip.gzhtcm.edu.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Arginine;
analogs & derivatives;
analysis;
Biological Factors;
analysis;
isolation & purification;
pharmacology;
Enzyme Assays;
methods;
Enzyme Inhibitors;
analysis;
isolation & purification;
pharmacology;
Hirudins;
analysis;
isolation & purification;
pharmacology;
Hirudo medicinalis;
chemistry;
Thrombin;
analysis;
antagonists & inhibitors
- From:
China Journal of Chinese Materia Medica
2010;35(15):1990-1992
- CountryChina
- Language:Chinese
-
Abstract:
As a potent anticoagulant, leech a traditional Chinese medicine, has become increasing topics. Hirudin, which is the primary effective component in leech, is a specific and efficient inhibitor of thrombin, mainly used in prevention and treatment of thrombus on the clinic practice. However, there is still no accurate and convenient method reported about the determination of it's biological activity. This paper reported a method for the determination of the biological activity the of extract from hirudo. The extra thrombin, which was not inhibited by hirudin in the extract from hirudo, reacted with N-benzoyl-L-arginine ethyl ester and was determined. The biological activity of the hirudo extract was determined, indirectly. The linear of calibration curve and accuracy were both perfect, the method was accurate and reliable.
