Effect of tectorigenin on MCP-1 and ICAM-1 mRNA expression in injured vascular endothelial cells.
- Author:
Jinfeng WANG
1
;
Yanping ZHANG
;
Cuiyan YANG
;
Mingxiao HOU
;
Fang WANG
;
Guoyu WANG
;
Shuhong WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Atherosclerosis; drug therapy; genetics; metabolism; Cells, Cultured; Chemokine CCL2; genetics; metabolism; Disease Models, Animal; Endothelial Cells; drug effects; metabolism; Gene Expression; drug effects; Humans; Intercellular Adhesion Molecule-1; genetics; metabolism; Isoflavones; pharmacology; RNA, Messenger; genetics; metabolism; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2010;35(15):2001-2003
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the protective effect of tectorigenin on the vascular endothelial cells(VEC) injured by oxidant low density lipoprotein-cholesterol and the expression of MCP-1 and ICAM-1 mRNA, and explore the mechanism of anti-atherosclerosis.
METHODThe VEC of rat was cultured in vitro and the 100 mg x L(-1) ox-LDL inducing oxidant injured model was used in this study. Different dosage tectorigenin was added into VEC and the activity of VEC was observed by MTT colorimetry. The expression of MCP-1 and ICAM-1 mRNA in VEC was detected by RT-PCR.
RESULTTectorigenin had significantly protective effect on the VEC injured by ox-LDL and obviously inhibited the excessive expression of MCP-1 and ICAM-1 mRNA in VEC.
CONCLUSIONIt was the critical mechanism of anti-atherosclerosis that tectorigenin prevented the VEC oxidant injured and inhibited the excessive expression of MCP-1 and ICAM-1.
