Influence of tongxie prescription on CRF expression in spinal cord and brain of hypersensitive viscera rats.
- Author:
Guanqun CHAO
1
;
Bin LV
;
Lina MENG
;
Shuo ZHANG
;
Lu ZAHNG
;
Yun GUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; drug effects; metabolism; Cerebral Ventricles; drug effects; metabolism; Corticotropin-Releasing Hormone; genetics; metabolism; Disease Models, Animal; Drugs, Chinese Herbal; administration & dosage; Gene Expression; drug effects; Humans; Irritable Bowel Syndrome; drug therapy; genetics; metabolism; Male; Random Allocation; Rats; Rats, Sprague-Dawley; Signal Transduction; drug effects; Spinal Cord; drug effects; metabolism
- From: China Journal of Chinese Materia Medica 2010;35(15):2012-2016
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the distribution and expression of corticotropin releasing factor (CRF) in spinal cord, hypothalamus and third ventricle of cerebrum, of the hypersensitive viscera rats, and to research on the mechanism of CRF in the hypersensitive vicera signal conduction pathway in IBS (irritable bowel syndrome) and to investigate possible active mechanisms of tongxie prescription on IBS.
METHODForty SD rats were divided randomly into three groups. The rats of model No. 1 were sensitized by injecting egg albumin into abdominal cavity. The rats of model No. 2 were sensitised by conditional stimulus and unconditional stimulus. The two model groups were both divided randomly into two groups. The five groups were given intragastric administration with Tongxie prescription or normal saline for four weeks. Then quantitative analysis of CRF in the lumbosacral spinal cord and brain of rats were achieved by immunohistochemical method and computerized image system.
RESULTThe sensitivity of the groups being treated with tongxie prescription were much lower than the model groups (P < 0.01). The immunohistochemical method showed that CRF was expressed in the lumbar intumescentia of spinal cord, hypothalamus and the diaphragmatic surface of third ventricle of cerebrum. The CRF positive index of the model groups was higher than that of the blank group (P < 0.01). The CRF positive index of the healing groups was lower than that of the model groups (P < 0.01). The CRF positive index of the healing groups was higher than that of the blank group (P < 0.01).
CONCLUSIONThe two model rats evoked by two different stimulation both appeared visceral hypersensitivity state. CRF is related to stress. The increase of CRF expression in the lumbar intumescentia of spinal cord, hypothalamus and the diaphragmatic surface of third ventricle of cerebrum showed that CRF is critical to the introduce of stimulus signal of vicera. Tongxie prescription can significantly decrease CRF expression. This is one mechanism to decrease sensitivity of hypersensitive viscera rats.
