Molecular authentication of medicinal Epimedium species--a case of diagnostic PCR authentication of Epimedium koreanum.

Author: Chuanyi WANG ¹ ; Yun LIANG ; Baolin GUO ; Peigen XIAO
Author Information

1. Chinese Academy of Medical Sciences & Peking Union Medical College Institute of Medicinal Plant Development, Beijing 100094, China.
Publication Type:Journal Article
MeSH: Alleles; China; DNA Primers; genetics; Epimedium; genetics; Molecular Sequence Data; Polymerase Chain Reaction; methods; Quality Control; Species Specificity
From: China Journal of Chinese Materia Medica 2010;35(14):1799-1802
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo establish the allele-specific diagnostic PCR methods for Epimedium koreanum.
METHODITS of eight medicinal Epimedium species were sequenced and downloaded from GenBank, and aligned to find the specific sites in E. koreanum. The diagnostic primers were designed based on the sites, and used to amplify all the samples, then verified with the crude drugs and decoction pieces from market.
RESULT AND CONCLUSIONTwo pairs of allele-specific diagnostic primers were successfully used to indentify E. koreanum from other medicinal species, and also can be used to identify the crude drugs and decoction pieces.