Cloning and expression of a new glucoamylase gene.
- Author:
Li-Quan YANG
1
;
Xiao-Jun DAI
;
Yuan-Ming LUO
;
Chun-Xiao MA
;
Jian-Hu HOU
;
Zhi-Qiang WU
;
Cui-Yan WANG
;
Ming-Gang LI
Author Information
1. The Key Laboratory of Bioactive Material, Ministry of Education, Life Science College, Nankai University, Tianjin 300071, China.
- Publication Type:Journal Article
- MeSH:
Biocatalysis;
Blotting, Western;
Cloning, Molecular;
Electrophoresis, Polyacrylamide Gel;
Fungal Proteins;
genetics;
metabolism;
Gene Expression Regulation, Enzymologic;
Gene Expression Regulation, Fungal;
Glucan 1,4-alpha-Glucosidase;
genetics;
metabolism;
Molecular Sequence Data;
Pichia;
genetics;
Recombinant Proteins;
metabolism;
Rhizopus;
enzymology;
genetics;
Sequence Analysis, DNA
- From:
Chinese Journal of Biotechnology
2007;23(3):477-524
- CountryChina
- Language:Chinese
-
Abstract:
According to the reported gene sequence of Rhizopus oryzae glucoamylases, the glucoamylase gene containing four introns was cloned from the total DNA of the natural Rhizopus arrhizu. Specific primers were designed to delete introns by overlapping PCR and a new cDNA sequence of Rhizopus arrhizu glucoamylase was obtained. The accession number in gene bank is DQ903853. This gene is successfully expressed in the Picha pastoris, producing a new protein with a high activity of glucoamylase.
