Preparation and performance of Chitosan-oligosaccharides/DNA complex nanoparticles.
- Author:
Jun-Lü QIAN
1
;
Rong-Rong ZHU
;
Jin HUANG
;
Shi-Long WANG
;
Xiao-Yu SUN
Author Information
1. Department of Chemistry of Tongji University, Shanghai 200092, China.
- Publication Type:Journal Article
- MeSH:
Chitosan;
chemistry;
DNA;
chemistry;
Gene Transfer Techniques;
Genetic Vectors;
Nanoparticles;
chemistry;
Oligosaccharides;
chemistry
- From:
Chinese Journal of Biotechnology
2007;23(4):741-745
- CountryChina
- Language:Chinese
-
Abstract:
Comparing to Chitosan, Chitosan-oligosaccharides have several special functions, such as water-soluble, antitumor activity, immunostimulating effects, and antimicrobial activity. The chitosan-oligosaccharide, the molecular weight of which was about 5000, was used as research model. According to the agarose gel electrophoresis and UV spectrophotometer it was proved that electrostatic interaction was playing a very important role in the formation process of chitosan-oligosaccharide/DNA complex. The potential of adsorbing DNA on chitosan-oligosaccharide was analyzed by gel electrophoresis and UV spectrophotometer, and it was indicated that chitosan-oligosaccharide can improve the storage and structure stability of DNA. To check its protection ability to DNA by DNase I digestive experiment, the result showed that chitosan-oligosaccharide could load with plasmid effectively and protect DNA from being digested by DNase I. It was proved that chitosan-oligosacchide was safe and effective for gene delivery and will have a very good future in the field of gene therapy.
