Study on differentially expressed proteins of effect of kudiezi injection on cerebral cortexin rats with cerebral ischemic stroke and heat toxin syndrome.
- Author:
Feng-Li WANG
;
Yun-Ling ZHANG
;
Xue-Mei LIU
;
Xin-Xiang WANG
;
Hong ZHENG
;
Xin-Yang ZHANG
;
Fang GAO
;
Ting YAO
- Publication Type:Journal Article
- MeSH:
Animals;
Brain Ischemia;
drug therapy;
genetics;
metabolism;
pathology;
Cerebral Cortex;
drug effects;
metabolism;
pathology;
Drugs, Chinese Herbal;
administration & dosage;
Gene Expression;
drug effects;
Humans;
Male;
Rats;
Rats, Sprague-Dawley;
Stroke;
drug therapy;
genetics;
metabolism;
pathology
- From:
China Journal of Chinese Materia Medica
2014;39(10):1874-1879
- CountryChina
- Language:Chinese
-
Abstract:
This study is to investigate the modulation of Kudiezi (KDZ) injection on differential protein expression in cerebral cortex of rats with cerebral ischemic stroke and heat toxin syndrome established by intraperitoneal injection of carrageenan and middle cerebral artery occlusion (MCAO) methods. According to random number table rats were divided into three groups: drug group, model group and sham group. The tripheye tetrazolium chloride (TTC) staining and HE staining were used to observe brain tissue injury of rats. After therapeutic intervention with above drug for seventy-two hours, the level of differential protein expression was analyzed by two-dimensional gel electrophoresis (2-DE). The results show that there are differential protein expressions between cerebral ischemic stroke and heat toxin syndrome rats and sham rats. Furthermore, as a Chinese medicine injection with effect of clearing heat, resolving toxin and dredging collaterals, KDZ injection can decrease alleviate morphological changes of cerebral ischemia, regulate the levels of some differential proteins expression.
