Effects of different processing methods on effective components and sulfur dioxide residue in Gastrodiae Rhizoma.
- Author:
Zi-Wan NING
;
Chun-Qin MAO
;
Tu-Lin LU
;
De JI
;
Jing LIU
;
Lin JI
;
Huan YANG
;
Fa-Qin WANG
- Publication Type:Journal Article
- MeSH:
Drug Contamination;
Drugs, Chinese Herbal;
chemistry;
Gastrodia;
chemistry;
Sulfur Dioxide;
analysis;
Technology, Pharmaceutical;
methods
- From:
China Journal of Chinese Materia Medica
2014;39(15):2814-2818
- CountryChina
- Language:Chinese
-
Abstract:
The contents of adenosine, gastrodin, 4-hydroxybenzyl alcohol, 4-hydroxybenzaldehyde, parishin and sulfur dioxide residue were compared in differently-processed Gastrodiae Rhizoma to provide the basis for a reasonable processing method of Gastrodiae Rhizoma. The analysis was performed on a Merck Purospher STAR column (4.6 mm x 250 mm, 5 μm) with a mobile phase consisting of methanol and water (containing 0.1% formic acid) under gradient elution at a flow rate of 1.0 mL x min(-1). The eluates were detected at 270 nm, and the column temperature was 35°C. The content of adenosin, gastrodin, 4-hydroxybenzyl alcohol, 4-hydroxy-benzaldehyde and parishin in processing of boiling or sulfur-fumigated were lower than that of in processing of steaming. Furthermore, the sulfur dioxide residue of sulphur-fumigated groups exceed 400 mg x kg(-1). This stable and reliable method will contribute to the quality control of different processed Gastrodiae Rhizoma.
