Optimization of extraction process for tannins from Geranium orientali-tibeticum by supercritical CO2 method.
- Author:
Song XIE
;
Zhi-Ping TONG
;
Rui TAN
;
Xiao-Zhen LIU
- Publication Type:Journal Article
- MeSH:
Carbon Dioxide;
chemistry;
Chemical Fractionation;
methods;
Drugs, Chinese Herbal;
chemistry;
Geranium;
chemistry;
Tannins;
isolation & purification
- From:
China Journal of Chinese Materia Medica
2014;39(15):2912-2914
- CountryChina
- Language:Chinese
-
Abstract:
In order to optimize extraction process conditions of tannins from Geranium orientali-tibeticum by supercritical CO2, the content of tannins was determined by phosphomolybdium tungsten acid-casein reaction, with extraction pressure, extraction temper- ature and extraction time as factors, the content of tannins from extract of G. orientali-tibeticum as index, technology conditions were optimized by orthogonal test. Optimum technology conditions were as follows: extraction pressure was 25 MPa, extraction temperature was 50 °C, extracted 1.5 h. The content of tannins in extract was 12.91 mg x g(-1), extract rate was 3.67%. The method established could be used for assay the contents of tannin in G. orientali-tibeticum. The circulated extraction was an effective extraction process that was stable and feasible, and that provides a way of the extraction process conditions of tannin from G. orientali-tibeticum.
