Study on quantitative methods of cleistocalycis operculati cortex.
- Author:
Li-Si CHEN
;
Jia-Ju OU
;
Shu-Yuan LI
;
Song-Gui LU
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Chromatography, Reverse-Phase;
methods;
Drugs, Chinese Herbal;
analysis;
Plant Bark;
chemistry;
Syzygium;
chemistry
- From:
China Journal of Chinese Materia Medica
2014;39(16):3128-3130
- CountryChina
- Language:Chinese
-
Abstract:
Cleistocalycis Operculati Cortex is the dry bark of Cleistocalyx operculatus. It is the raw material of Compound Hibiscuse which is external sterilization antipruritic drugs. The quality standard of Cleistocalycis Operculati Cortex in Guangdong Province "standard for the traditional Chinese medicine" (second volumes) only contains TLC identification. It is unable to effectively monitor and control the quality of Cleistocalycis Operculati Cortex. A reversed-phase HPLC method was established for the determination of 3, 3'-O-dimethylellagic acid from Cleistocalycis Operculati Cortex and the content was calculated by external standard method for the first time. Under the selected chromatographic conditions, the target components between peaks to achieve effective separation. 3,3'-O- dimethylellagic acid standard solution at the concentration of 1.00 - 25.0 mg x L(-1) showed a good linear relationship. The standard curve was Y = 77.33X + 7.904, r = 0.999 5. The average recovery was 101.0%, RSD was 1.3%. The HPLC method for the determination of 3,3'-O-dimethylellagic acid in Cleistocalycis Operculati Cortex is accurate and reliable. It can provide a strong technical support for monitoring the quality of Cleistocalycis Operculati Cortex.
