PEI-FeONanoparticles for Human Amniotic Epithelial Cells Labeling
10.3881/j.issn.1000-503X.2017.05.003
- Author:
Dian-Bao ZHANG
1
;
Ying LI
1
;
Hang SU
2
;
Jing WANG
2
;
Ping SHI
3
;
Xi-Ning PANG
1
Author Information
1. Department of Stem Cells and Regenerative Medicine,College of Basic Medical Sciences,Shenyang 110122,China.
2. Department of Anus & Intestine Surgery,the First Clinical College,Shenyang 110122,China.
3. Department of General Practice,the First Clinical College,China Medical University,Shenyang 110122,China.
- Publication Type:Journal Article
- From:
Acta Academiae Medicinae Sinicae
2017;39(5):611-614
- CountryChina
- Language:English
-
Abstract:
Objective To label human amniotic epithelial cells(hAECs) by using PEI-FeOnanoparticles. Methods The PEI-FeOnanoparticles were characterized by using transmission electron microscopy and dynamic light scattering. The primary cultured hAECs were labeled with the nanoparticles,and the labeling efficiency was evaluated by Prussian blue staining. The cell survival rate and viability were tested by using placenta blue staining and CCK-8 assay,respectively. Results The PEI-FeOnanoparticles were compact spheres with an average particle size of 13 nm,a hydrodynamic radius of 17.56 nm,and a zeta potential of+34.5 mV. The labeling efficiency of the nanoparticles on hAECs reached 91% when the concentrations were greater than 20 μg/ml. When the concentrations of nanoparticles were at 50 μg/ml(t=16.37,P<0.0001;t=10.39,P<0.0001) and 100 μg/ml(t=29.89,P<0.0001;t=16.86,P<0.0001),the cell survival rates and cell viabilities were significantly reduced versus controls. Conclusion The PEI-FeOnanoparticles can be used for labeling hAECs without obvious cytotoxicity at its working concentration.
