In Vitro Efficacy of Continuous Mild High Temperature on the Biofilm Formation of Aspergillus Niger.
10.3881/j.issn.1000-503X.2017.06.005
- VernacularTitle:可耐受高温对黑曲霉生物膜体外模型构建的影响
- Author:
Rong ZENG
1
;
Jian Bo TONG
1
;
Yu Zhen LIU
1
;
Qing CHEN
2
;
Tong LIN
1
;
Min LI
3
;
Gui Xia LÜ
1
Author Information
1. Institute of Dermatology,CAMS and PUMC,Nanjing 210042,China.
2. Jiangsu Province Blood Center,Nanjing 210042,China.
3. Jiangsu Key Laboratory of Molecular Biology for Skin Diseases & STDs,Nanjing 210042,China.
- Publication Type:Journal Article
- From:
Acta Academiae Medicinae Sinicae
2017;39(6):762-767
- CountryChina
- Language:English
-
Abstract:
Objective To investigate whether continuous mild high temperature (increased temperature without causing significant damage to host cells) can inhibit the biofilm formation of Aspergillus niger (A.niger) and its vitality.Methods A.niger biofilms were formed on a coverslip in 24-well tissue culture plate and were checked at the time points 4,8,10,16,24,48 and 72 hours.Confocal laser scanning microscopy (CLSM) was used to image and quantify A.niger biofilm formation under three different continuous mild high temperatures at 37℃,39℃,and 41℃.Furthermore,2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay was used to quantify the dynamic growth of A.niger biofilm under the above conditions.Results Compared with the culture condition 37℃,CLSM analysis at 39℃ or 41℃ showed that higher temperature induced later germination at 4 hours (t=8.603,P=0.047;t=14.550,P=0.008),poorer hyphal elongation at 8 hours(t=35.118,P=0.039;t=63.450,P=0.006),poorer polar growth,and reduced biofilm thickness from 10 to 24 hours.The XTT assay showed that higher temperature (39℃ or 41℃) lead to lower vitality at 10 hours,higher vitality at 16 hours,but finally lower vitality from 24 to 72 hours (t=24.262,P=0.038;t=7.556,P=0.031).Conclusion Continuous mild high temperature may have a negative regulatory effect on biofilm formation of A.niger and its vitality.
