Molecular analysis on non-O1 and non-O139 Vibrio cholerae isolates
10.3760/cma.j.issn.0254-6450.2012.12.015
- VernacularTitle:非O1/非O139群霍乱弧菌感染病例分离株分子流行病学分析
- Author:
Dao-Li CHEN
1
;
Ping ZHANG
;
Duo-Chun WANG
;
Jin CHEN
;
Bai-Qi YU
;
Xian-Feng CHENG
;
Bao-Wei DIAO
;
Hai-Jian ZHOU
;
Ming ZHU
;
Wan-Fu HU
;
Sheng-Wei ZHAN
;
Huai-Qi JING
;
Biao KAN
Author Information
1. 243000,安徽省马鞍山市疾病预防控制中心
- Keywords:
Vibrio cholerae non-O1/non-O139;
Real-time PCR;
Virulence gene;
Pulsed-field gel electrophoresis
- From:
Chinese Journal of Epidemiology
2012;33(12):1265-1268
- CountryChina
- Language:Chinese
-
Abstract:
Objective According to results from the two-month consecutive surveillance program in Maanshan,six suspected cases of non-O1 non-O139 Vibrio (V.) cholerae infection,were found that called for identification of pathogens as well as molecular-epidemiological analysis to determine the aggregation of the epidemic situation.Methods Biochemical and serotype identification,hemolysis test,and drug sensitive test were used to detect the drug resistance spectrum.Real-time PCR and conventional PCR were used to detect the presence of V.cholerae specific genes,virulent genes and its related genes,including ompW,ctx,tcpA,toxR,hlyA,zot,ace,rstR and g ⅢCTX.Pulsed-field gel electrophoresis (PFGE) was used to analyze the molecular type of strains.Results All the six isolates of non-O 1 non-O 139 V.cholerae were identified by biochemical and serologic tests,and appeared to be β hemolytic.Twelve out of the 14 kinds of drugs showed 100％ sensitive.All isolates were positive of ompW gene by real-time PCR,but negative for ctx,tcpA,zot,ace,rstR and gⅢ CTK.Five of the six isolates were positive for toxR and hlyA,except for strain 1001434446.All strains had different PFGE types,but two strains had similar types.All strains had a low similarity compared to the toxigenic V.cholerae.Conclusion Six cases ofnon-O1 and non-O139 nontoxigenic V.cholerae infection appeared in the same period.Along with epide(m)iological information,we noticed that these cases had a sporadic nature,but frequently appeared in the same area.We got the impression that public health measurements should be strengthened,with special attention paid to those diarrhea outbreaks caused by non-O 1 /non-O 139 strains since V.cholerae had appeared in low incidence.
