Analysis on Mycoplasma pirum infection in male HIV/AIDS patients and related 16S rRNA genes in Jiangsu province
10.3760/cma.j.issn.0254-6450.2013.03.013
- VernacularTitle:江苏省男性HIV感染者梨支原体16S rRNA基因片段序列分析
- Author:
Jian-Ru WU
1
;
Yi ZHU
;
Yan-Xin XIE
;
Liang-Jia ZHOU
;
Jin-Shui XU
;
Xi-Ping HUAN
;
Bei WANG
Author Information
1. 东南大学公共卫生学院流行病与卫生统计学系
- Keywords:
Mycoplasma pirum;
HIV/AIDS;
p 1 gene;
16S rRNA gene
- From:Chinese Journal of Epidemiology
2013;34(3):259-262
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the prevalence ofMycoplasma pirum (Mpi) in male HIV infected patients,and to identify the 16S rRNA gene of Mpi.Methods The first void urine of male HIV/AIDS patients in Jiangsu province was collected for Mpi detection.Purified 16S rRNA gene PCR production was sequenced for analysis on its identification,homogeneity and phylogenetic tree.P1 protein sequence of Mpi was analyzed by Vector NTI Advance 11.0 to calculate the coded amino acid sequence.Homogeneity analysis was conducted between the theoretical amino acid sequence of Mpi and other Mycoplasmas.Results The prevalence of Mpi in male HIV/AIDS patients was 21.5％while the Mpi prevalence rates in different age groups were significantly different (x2Mpi=124.63,P＜0.01).The homogeneity of 18 strains of Mpi was higher than 90％.Conclusion The Mpi prevalence seemed much higher than the results from previous detection on HIV/AIDS patients,suggesting that more attention should be paid on AIDS treatment.More bioinformatic research on gene/nucleotide sequence analysis and forecast should be carried out to identify the molecular characteristics of Mpi.
