Effect of xiaotan sanjie recipe on expressions of VEGF-C and VEGFR-3 in nude mice with transplanted human gastric adenocarcinoma cell MKN-45.
- Author:
Bin PANG
1
;
Pin-Kang WEI
;
Yong-Jin LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Drugs, Chinese Herbal; pharmacology; Humans; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Stomach Neoplasms; metabolism; pathology; Vascular Endothelial Growth Factor C; metabolism; Vascular Endothelial Growth Factor Receptor-3; metabolism; Xenograft Model Antitumor Assays
- From: Chinese Journal of Integrated Traditional and Western Medicine 2011;31(2):204-208
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the mechanism of Xiaotan Sanjie Recipe (XSR) in inhibiting lymphatic metastasis of gastric carcinoma by observing its effects on the expressions of vascular endothelial growth factor-C (VEGF-C) and its receptor VEGFR-3 in nude mice with transplanted human gastric adenocarcinoma cell MKN-45.
METHODSThirty mice were made into MKN-45 tumor model and randomly divided into three groups treated with saline, 5-Fu, and XSR, respectively. Gene and protein expressions of VEGF-C and VEGFR-3 in the tumor tissue were detected by RT-PCR, and the lymphatic microvessel density (LMVD) in tumor was measured with immunohistochemistry.
RESULTSCompared with the group treated with saline, the mRNA and protein expressions of VEGF-C and VEGFR-3 as well as LMVD level were significantly lower (P < 0.05 or P < 0.01) in the group treated with XSR. There was no significantly statistic difference between the group of XSR and 5-Fu on the indices mentioned above (P > 0.05).
CONCLUSIONBy down-regulating the mRNA and protein expressions of VEGF-C and VEGFR-3 might be one of possible mechanisms for XSR in preventing and curing the lymphatic metastasis of gastric carcinoma.
