The effects of chitosan on different-derived fibroblasts.
- Author:
Jing-De ZHANG
1
;
Xin XING
;
Mei-qing SUN
;
Jian-xing SONG
;
Tian-xiang OUYANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Cell Division; drug effects; Chitin; analogs & derivatives; pharmacology; Chitosan; Dose-Response Relationship, Drug; Female; Fibroblast Growth Factors; secretion; Fibroblasts; drug effects; metabolism; ultrastructure; Hemostatics; pharmacology; Humans; Interleukin-8; secretion; Male; Microscopy, Electron; Peptide Fragments; secretion; Transforming Growth Factor beta; secretion
- From: Chinese Journal of Plastic Surgery 2004;20(1):53-56
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of chitosan on the biological activities of the fibroblasts derived from different tissues.
METHODSThe biological activities of the fibroblasts derived from different tissues were evaluated with a MTT method for fibroblast proliferation, photic and electronic microscope for morphologic and subcellular structure, 3H-proline uptake method for collagen secretion and ELISA box for the secretion of TGF-beta 1, FGF-AB, and IL-8.
RESULTSThis study showed that the chitosan inhabited the proliferation of the fibroblasts and the secretion of the TGF-beta 1, FGF-AB and collagen of the fibroblasts with a dose-depended manner in the normal skin, hypertrophic scar and keloid groups, but it stimulated the IL-8. However, there were no significant differences among the three groups (P > 0.05).
CONCLUSIONThe chitosan could inhibit the growth, proliferation, biosynthesis and secretion of the fibroblasts, and it may be used to treat different scars.
