An experiment study on repair of peripheral nerve defects by GDNF gene modified Schwann cells.
- Author:
Ping PING
1
;
Qing-feng LI
;
Di-sheng ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Glial Cell Line-Derived Neurotrophic Factor; Immunohistochemistry; Male; Nerve Growth Factors; analysis; genetics; physiology; Nerve Regeneration; physiology; Peripheral Nerves; chemistry; physiopathology; Peripheral Nervous System Diseases; surgery; Rats; Rats, Wistar; Schwann Cells; metabolism; transplantation
- From: Chinese Journal of Plastic Surgery 2003;19(5):369-372
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate an effective treatment of peripheral nerve injuries by means of gene transference.
METHODS48 adult Wister rats were divided evenly into 3 groups. A 10 mm sciatic nerve gap was created and bridged with a silicone chamber. The silicone chamber was filled with glial cell-line derived neurotrophic factor(GDNF) gene modified Schwann cells(SCs) (group 1), the normal SCs(group 2) and nothing(the control). At 4, 8, 12, and 16 weeks after the operation, the general and histological observations, the electromyographic and immunohistochemical examinations were performed to the regenerated nerves.
RESULTSThe GDNF-SCs group was significantly better than the SCs and the control groups in nerve conduction velocity, the number and density of reinnervation, the area of regenerated nerve and the thickness of myelin sheath of the regenerated nerves.
CONCLUSIONGDNF gene modified SCs secrete higher levels of neurotrophic factors for a prolonged time, which are more effective in peripheral nerve repair than the normal SCs.
