Collagen synthesis and expression of connective tissue growth factor in the cultured fibroblasts of human hypertrophic scar.
- Author:
Shi-rong LI
1
;
Jian-yi LIU
;
Shu-xing JI
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Cicatrix, Hypertrophic; genetics; metabolism; pathology; Collagen; biosynthesis; Connective Tissue Growth Factor; Fibroblasts; metabolism; pathology; Gene Expression; Humans; Immediate-Early Proteins; genetics; Immunohistochemistry; Intercellular Signaling Peptides and Proteins; genetics; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Plastic Surgery 2004;20(2):124-127
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of connective tissue growth factor on the pathogenesis of human hypertrophic scar.
METHODSNormal skin and hypertrophic scar fibroblasts were cultured in vitro. The collagen synthesis of fibroblasts were measured by H3-proline incorporation method. The expression of connective tissue growth factor protein and mRNA of fibroblasts were detected with immunocytochemistry staining and reverse transcription polymerase chain reaction methods.
RESULTSCompared with normal skin fibroblast, the collagen synthesis and the expression of connective tissue growth factor protein and mRNA in the hypertrophic scar fibroblast was higher (P < 0.01).
CONCLUSIONConnective tissue growth factor may play an important role in promoting the fibrotic process of hypertrophic scar.
