Re-growth of induced-osseous tissue encapsulated in muscular fasciae for prefabrication of an osteo-musculo-cutaneous flap.
- Author:
Yu ZHAO
1
;
Ping HU
;
Ying-ling LU
;
Feng GAO
;
Qun QIAO
;
Ke-ming QI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Fascia; transplantation; Histocytochemistry; methods; In Situ Hybridization; Male; Mice; Mice, Nude; Models, Animal; Osteonectin; genetics; metabolism; RNA, Messenger; genetics; metabolism; Staining and Labeling; Stem Cell Transplantation; methods; Surgical Flaps; blood supply; Tissue Engineering; methods
- From: Chinese Journal of Plastic Surgery 2004;20(2):132-135
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo improve the survival of mesenchymal stem cells (MSCs) for prefabrication of an osteo-musculo-cutaneous flap.
METHODSIn a mouse model, the compound of MSCs and p(3HB-co-3HH) were embeded in the latissimus dorsi muscle as an experimental group and the muscle pocket of the buttock as the control. The examinations of the HE staining, hybridization in situ of osteonectin mRNA and Von kossa staining were used to evaluate the results.
RESULTSThe expression of osteonectin mRNA and the Von kossa staining showed that the latissimus dorsi muscle group was superior to the control in 2 weeks and 4 weeks after the surgery in vivo.
CONCLUSIONThe results indicate that the above-mentioned technique may be a good alternative for the prefabrication of the osteo-musculo-cutaneous flap.
