The TGF-alpha gene Taq I polymorphism and non-syndromic cleft lip with or without cleft palate.
- Author:
Wen-guang ZHANG
1
;
Shao-jun LUO
;
Shao-ming TANG
;
Jie LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Child; Child, Preschool; China; Cleft Lip; genetics; Cleft Palate; genetics; DNA; genetics; metabolism; Female; Gene Frequency; Genotype; Humans; Infant; Male; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Taq Polymerase; metabolism; Transforming Growth Factor alpha; genetics
- From: Chinese Journal of Plastic Surgery 2004;20(3):190-193
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the association of TGF-alpha gene Taq I polymorphism and nonsyndromic cleft lip with or without cleft palate (NSCL/P) in Chinese.
METHODS107 patients with NSCL/P and 136 healthy controls were examined for TGF-alpha/Taq I genotypes. TGF-alpha/Taq I typing was carried out by digesting the locus specific polymerase chain reaction amplified products with alleles specific Taq I restriction enzyme (PCR-RELP).
RESULTSThe C2 allele frequency of TGF-alpha/Taq I in patients with NSCL/P (16%) was significantly higher than that in healthy controls (8%). The C2 genotype frequency of TGF-alpha/Taq I in NSCL/P patients with positive family history (12.5%) was significantly higher than that in healthy controls.
CONCLUSIONThese findings demonstrate the role of TGF-alpha as a gene of major effects in the development of nonsyndromic cleft lip with or without cleft palate clefts in human. These findings suggest that a family history of clefting may correlate with the TGF-alpha Taq I rare variation.
