The role of tyrosine phosphorylation proteins in procollagen gene expression of fibroblasts in wound healing.
- Author:
Min-liang CHEN
1
;
Jia-ke CHAI
;
Hui-feng SONG
;
Yan-qiu WU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Benzoquinones; Blotting, Western; Child; Female; Fibroblasts; metabolism; Gene Expression; Humans; Lactams, Macrocyclic; Male; Phosphoproteins; metabolism; physiology; Phosphorylation; drug effects; Procollagen; genetics; Quinones; pharmacology; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Rifabutin; analogs & derivatives; Skin; drug effects; metabolism; pathology; Tyrosine; metabolism; Wound Healing; genetics; physiology
- From: Chinese Journal of Plastic Surgery 2004;20(3):212-214
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of adhesion between fibronectin and fibroblasts in wound healing as well as tyrosine phosphorylation proteins in procollagen mRNA expression.
METHODSThe level of proalpha1 (I) mRNA and tyrosine phosphorylation protein were detected employing the techniques of RT-PCR and immunoblotting. After inhibition of tyrosine kinases, herbimycin A was added to the medium to block the pathway of tyrosine phosphorylation, the changes of procollagen mRNA and tyrosine phosphorylation proteins were further investigated.
RESULTSThe adhesion between fibroblasts and fibronectin in wound healing not only induced the production of 98kd and 65kd tyrosine phosphorylation protein, but also enhanced obviously the expression of procollagen alpha1 (I) mRNA. When the pathway of tyrosine phosphorylation was blocked, the level of procollagen alpha1 (I) mRNA lowered remarkably, accompanied by the decrease of 98kd, 65kd tyrosine phosphorylation proteins.
CONCLUSIONThe adhesion between fibronectin and fibroblasts plays an important role in expression increase of procollagen mRNA during wound healing, in the process of which tyrosine phosphorylation is a key step.
