Preparation of contraceptive pill microcapsule and its anti-fertility effect.
- Author:
Zhongqiong YIN
1
;
Renyong JIA
;
Ping GAO
;
Rong GAO
;
Dehong JIANG
;
Kun LIU
;
Shigui LIU
Author Information
1. College of Life Science, Sichuan University, Chengdu 610064, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Capsules;
Contraceptive Agents, Male;
chemical synthesis;
pharmacology;
Drug Compounding;
Glycerides;
chemistry;
Male;
Mice;
Palmitic Acids;
isolation & purification;
pharmacology;
Particle Size;
Sperm Motility;
drug effects;
Spermatogenesis;
drug effects;
Stearic Acids;
isolation & purification;
pharmacology;
Terpenes;
chemistry
- From:
Journal of Biomedical Engineering
2004;21(6):979-982
- CountryChina
- Language:Chinese
-
Abstract:
The main component of this pill is 2-Octadecanoic acid-4-Palmitic acid-2, 4-Pentanediyl ester separated from chloroform extract of neem oil. The microcapsules coated by the re-curdle method were fabricated with an average particle size of 100-180 microm. The morphological characteristics, incorporation efficiency, carrier reclamation efficiency of the microcapsule were investigated. Kunming mice were used in the experiment, and the anti-fertility effect of the microcapsule on the histology and apoptosis was studied by light and electron microscopy and the flow cytometry. The data obtained clearly indicated that the microcapsule could lead to the payload of medicine, the incorporation efficiency being 90%. After the microcapsules were given to the male mice orally, its anti-fertility effect came into being and could keep the mice in a state of reversible infertility for a long time. The results of histological study and flow cytometry indicate that the mechanism of its anti-fertility effect involves mainly the inhibition of sperm motility and the arrest of spermatogenic process.
