Genotype combination distribution of 5-site SNPs in 3 subtypes of beta adrenoceptor.
- Author:
Zhengxiang XIE
1
;
Yonghong NIU
;
Houxun MA
;
Zhangyong LI
;
Yuehui YIN
Author Information
1. Department of Biomedical Engineering in Chongqing University of Medical Sciences, Chongqing 400016, China. bmezxxie@public.cta.cq.cn
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Female;
Genotype;
Humans;
Male;
Middle Aged;
Polymerase Chain Reaction;
methods;
Polymorphism, Single Nucleotide;
genetics;
Receptors, Adrenergic, beta;
classification;
genetics;
Receptors, Adrenergic, beta-1;
genetics;
Receptors, Adrenergic, beta-2;
genetics;
Receptors, Adrenergic, beta-3;
genetics
- From:
Journal of Biomedical Engineering
2005;22(1):99-103
- CountryChina
- Language:Chinese
-
Abstract:
DNA was extracted from the peripheral venous blood of 338 subjects using BLOOD DNA MINI KIT. The 5 site SNP in 3 subtypes of Beta-AR were genotyped by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and allele-specific primer PCR techniques. The genotypes combination distribution of SNP at 5 sites in the 3 subtypes of Beta-AR were determined by clustering analysis technique. The natural combination distribution characteristics for SNP at 5 sites in the 3 subtypes of Beta-AR in 338 subjects were obtained. Sixty-seven combinations types were found. The preceding 5 combinations in the natural combination distribution of the SNP were: (1) The genotype combination of forty subjects was B1-AR S/S49+B1-AR R/R389+B2-AR R/G16+B2-AR Q/E27+B3-AR W/W64, its probability was 11.83%. (2) The genotype combination of thirty-three subjects was B1-AR S/S49+B1-AR R/R389+B2-AR R/G16+B2-AR Q/Q27+B3-AR W/W64, its probability was 9.76%. (3) The genotype combination of nineteen subjects was B1-AR S/S49+B1-AR R/G389+B2-AR R/G16+B2-AR Q/Q27+B3-AR W/W64, its probability was 5.62%. (4) The genotype combination of sixteen subjects was B1-AR S/S49+B1-AR R/G389+B2-AR R/G16+B2-AR Q/E27+B3-AR W/W64, its probability was 4.74%. (5) The genotype combination of thirteen subjects was B1-AR S/G49+B1-AR R/R389+B2-AR R/G16+B2-AR Q/E27+B3-AR W/W64, its probability was 3.85%. The obvious correlations exist among full sample and female or male subgroup, and between female and male subgroups.
