Interleukin-8 protein and gene expression in atherosclerotic lesions of hyperlipemia rabbits.
- Author:
Yongmei NIE
1
;
Huaiqing CHEN
;
Min CHENG
;
Xiaojing LIU
;
Yiling DENG
Author Information
1. Department of Biomedical Engineering, West China Medical Center of Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Aorta;
metabolism;
Atherosclerosis;
etiology;
metabolism;
Hyperlipidemias;
complications;
Interleukin-8;
biosynthesis;
genetics;
Male;
RNA, Messenger;
biosynthesis;
genetics;
Rabbits
- From:
Journal of Biomedical Engineering
2005;22(2):258-264
- CountryChina
- Language:Chinese
-
Abstract:
Interleukin-8 is CXC chemokine that is initially discovered using chemotaxis and the activation of neutrophils and induces the migration and proliferation of smooth muscle cells. Interleukin-8 is a potent angiogenic factor that may play a role in atherosclerosis. To establish the temporal correlation between IL-8 expression and plaque development, we examined the expression during atherosclerosis of hyperlipemia rabbits using immunohistochemistry, ELISA, in situ hybridization. By location of immunohistochemistry, the expression of IL-8 protein increased obviously in intima of hyperlipemia rabbits at 8 and 12 week. Quantitative analysis of the expression of IL-8 Immunohistochemistry indicated that positive area of AS model was 4.48 times and 8.76 times that of control group at 8 and 12 week. The valuation of IOD of AS model was 4.16 times and 4.36 times that of control group at 8 and 12 week. By specific ELISA, the ratio of the IL-8 protein to total protein of AS model was 1.84 times and 2.06 times that of control group at 8 and 12 week. By location of in situ hybridization, positive location was strong in intima of hyperlipemia rabbits at 8 week. We observed the dynamic alteration of interleukin-8 protein and gene expression in atherosclerotic lesions of hyperlipemia rabbits with establishing model. Interleukin-8 protein and gene expression was up-regulation in the development of fatty streaks in hyperlipemia rabbit.
