Protective effect of follicle stimulating hormone on apoptosis of human epithelial ovarian cancer cell induced by cisplatin.
- Author:
Chun-fang HUANG
1
;
Dong-yuan LIU
;
Wei-hua XU
;
Keng SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Cisplatin; pharmacology; Female; Flow Cytometry; Follicle Stimulating Hormone; pharmacology; Humans; Ovarian Neoplasms; chemistry; pathology; Receptors, FSH; analysis; Tumor Cells, Cultured
- From: Acta Academiae Medicinae Sinicae 2003;25(4):443-446
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the protective effects of follicle stimulating hormone (FSH) on human epithelial ovarian cancer cell apoptosis induced by cisplatin (DDP).
METHODSOVCAR3-FSHR cell were treated with DDP and FSH at serials of concentrations, MTT assay was used to examine the growth inhibition of OVCAR3-FSHR cell after treatment with DDP and FSH. Flow cytometry was used to analyze the change of cell cycle and percentage of apoptosis.
RESULTSIt was revealed that FSH decreased the growth inhibition induced by DDP. We also demonstrated that FSH reduced the S-phase percentage compared with the DDP only groups after treatment for 24 hours and reduced apoptosis percentage after 48 hours treatment with DDP.
CONCLUSIONIt is suggested that FSH can protect the apoptosis induced by DDP. It also suggests that FSH may be an important chemoresistent reason for the chemotherapy of ovarian cancer.
