Follicle stimulating hormone inhibits cisplatin induced apoptosis in ovarian cancer cells.
- Author:
Chun-fang HUANG
1
;
Dong-yuan LIU
;
Keng SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; antagonists & inhibitors; pharmacology; Apoptosis; drug effects; Caspase 3; Caspases; metabolism; Cisplatin; antagonists & inhibitors; pharmacology; DNA Fragmentation; Female; Flow Cytometry; Follicle Stimulating Hormone; pharmacology; Humans; Inhibitor of Apoptosis Proteins; Microtubule-Associated Proteins; metabolism; Neoplasm Proteins; Ovarian Neoplasms; metabolism; pathology; Proto-Oncogene Proteins c-bcl-2; metabolism; Receptors, FSH; metabolism; Tumor Cells, Cultured
- From: Acta Academiae Medicinae Sinicae 2003;25(4):447-450
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether the follicle stimulating hormone (FSH) can inhibit apoptosis in ovarian cancer cells induced by cisplatin (DDP) and its possible mechinism.
METHODSDNA fragmentation assay, (TdT-mediated dUTP nick end labling TUNEL), Western blot were used to analyze the changes in expression levels of Survivin and bcl-2 protein. The relative activity of caspase-3 was also determined.
RESULTS200 mIU/ml FSH could regulate down the percentage of apoptotic cells and DNA fragmentation induced by 5.0 micrograms/ml cisplatin, while 200 mIU/ml FSH increased Survivin protein expression but could't influence the expression of bcl-2 protein.
CONCLUSIONFSH can inhibit ovarian cancer cells apoptosis induced by cisplatin. The possible mechinism is up-regulation of Survivin expression and down-regulation of caspase activity.
