cDNAs cloning of SARS-CoV PUMC2 viral genome.
- Author:
Zheng FAN
1
;
Xin-yu TAN
;
Bin YIN
;
Ke ZOU
;
Ting WANG
;
Yan SHEN
;
An-ping NI
;
Chuan QIN
;
Jian-gang YUAN
;
Bo-qin QIANG
;
Xiao-zhong PENG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA, Complementary; genetics; DNA, Viral; genetics; Genome, Viral; Molecular Sequence Data; Nucleic Acid Amplification Techniques; RNA, Viral; genetics; Reverse Transcriptase Polymerase Chain Reaction; SARS Virus; genetics; isolation & purification; Sequence Analysis, DNA; Viral Proteins; genetics
- From: Acta Academiae Medicinae Sinicae 2003;25(5):499-503
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo get the cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain.
METHODSUsing the SARS-CoV PUMC2 strain genomic RNA as the template, the cDNA fragments were amplified by RT-PCR, the PCR products were further purified and ligated into the pGEM-T vector, and all the clones obtained were sequenced.
RESULTSThe cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain were obtained.
CONCLUSIONSThese cDNAs can be provided for the function study of SARS-CoV proteins and the construction of full-length infectious cDNA clone of SARS-CoV.
