Isolation and identification of SARS-coronavirus in nasal and throat swabs collected from clinically diagnosed SARS patients.
- Author:
An-ping NI
1
;
Zhong WANG
;
Yong LIU
;
Shi-de LIU
;
Ye-hua HAN
;
Yan SHEN
;
Bo-qin QIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Aged, 80 and over; Antibodies, Viral; blood; Female; Humans; Larynx; virology; Male; Middle Aged; Nasopharynx; virology; SARS Virus; immunology; isolation & purification; Severe Acute Respiratory Syndrome; immunology; virology; Specimen Handling
- From: Acta Academiae Medicinae Sinicae 2003;25(5):520-524
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate and identify SARS-coronavirus in nasal and throat swabs collected from clinically diagnosed severe acute respiratory syndrome (SARS) patients.
METHODSNasal and throat swab specimens were inoculated onto well of 24-well plate containing confluent monolayers of Vero and MRC-5 cells. Isolates were identified with serology, electron microscopy and genome sequence.
RESULTSOne hundred and fifty-eight nasal and throat swabs specimens from 79 SARS patients in Peking Union Medical College Hospital between April and May, 2003 were cultured for SARS-coronavirus. Cytopathic effect (CPE) was found in three nasal swab specimens inoculated in Vero cells. Acute and convalescent phase serum specimens collected from SARS patients were found with seroconversions and/or a fourfold or greater rises in indirect fluorescence antibodies (IgG and IgM) titers when the 3 isolates (infected Vero cells) were used as antigen. Coronavirus was observed in the culture supernatant by negative-stain electron microscopy. Genome sequence confirmed the isolates were SARS-coronavirus.
CONCLUSIONSThe 3 isolates from nasal and throat swabs samples collected from 79 clinically diagnosed SARS patients were SARS coronavirus.
