Cytotoxicity of MICA-reactive V delta 1 gamma delta T cells towards epithelial tumor cells.
- Author:
Jin QI
1
;
Ping PENG
;
Meng-hua DAI
;
Yong-hai LI
;
Lian-xian CUI
;
Wei HE
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Female; HeLa Cells; pathology; Histocompatibility Antigens Class I; biosynthesis; genetics; Humans; Immunotherapy, Adoptive; Lymphocytes, Tumor-Infiltrating; immunology; Male; Membrane Proteins; biosynthesis; genetics; Middle Aged; Ovarian Neoplasms; immunology; Receptors, Antigen, T-Cell, gamma-delta; immunology; Recombinant Proteins; biosynthesis; genetics; T-Lymphocytes, Cytotoxic
- From: Acta Academiae Medicinae Sinicae 2004;26(1):1-7
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo confirm whether human MHC class I chain-related A (MICA) induces the amplification of V delta 1 gamma delta tumor-infiltrating lymphocytes (TILs) in vitro and to identify the cytotoxicity of MICA-reactive V delta 1 gamma delta TILs towards epithelial tumor cells.
METHODSMICA protein was prokaryoticly expressed and purified by molecular cloning technology. The purified recombined MICA (rMICA) was used to induce V delta 1 gamma delta T cells from tumor tissues in vitro and the cytotoxicity of these V delta 1 gamma delta TILs were tested by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT).
RESULTSThe rMICA was expressed in prokaryocyte with pET30 as a vector. The immobilized rMICA protein could markedly induce the amplification of V delta 1 gamma delta T cells from tumor tissue in vitro. These V delta 1 gamma delta T cells showed strong cytolytic activities towards tumor cell lines expressing MICA.
CONCLUSIONThe MICA-reactive V delta 1 gamma delta T cell may be a candidate for adoptive cellular therapy of tumors.
