Protective immune mechanisms induced by cellular vaccine made of the erythrocytic Plasmodium yoelii.
- Author:
Jie YUAN
1
;
Bei XU
;
Bao-feng LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Protozoan; blood; Enzyme-Linked Immunosorbent Assay; Female; Histocompatibility Antigens Class I; blood; Immunoglobulin G; blood; Malaria; prevention & control; Malaria Vaccines; immunology; Mice; Mice, Inbred BALB C; Plasmodium yoelii; immunology; Random Allocation; Vaccination
- From: Acta Academiae Medicinae Sinicae 2004;26(1):47-51
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the characteristics of protective immunity against Plasmodium yoelii (P.y.) infection by asexual blood-stages cellular vaccine.
METHODSThe particulate vaccines were constructed by saponin or double-distilled-water lysed parasitic red blood cells and inoculated into BALB/c mice by intraperitoneal injection (i.p.). Each group was challenged by the lethal erythrocytic P.y. parasites, and then their parasitemia and survival rates were detected. Expressions of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) were detected by RT-PCR. ELISA showed the serum antibodies against the malaria challenge and their-subclasses. Special membrane protein was recognized by immunofluorescence assay.
RESULTSThe vaccination with saponified erythrocytic parasites protected the immunized mice against P.y. challenge, while double-distilled-water lysed vaccine did not (P < 0.01). This protection was characterized by the increase of both IFN-gamma/IgG2a and IL-4/IgG1. Meanwhile, MHC class I alpha chain molecule was recognized on the membrane of infected-erthythrocyte.
CONCLUSIONSaponified P.y. asexual blood-stage cellular vaccine has a significantly high protective immunity against this lethal P.y. malaria, and the immunity may be associated with the expression levels of IgG2a and IFN-gamma. MHC class I alpha chain on infected erythrocytes may play an important role in the successful immunization.