Chromatin immunoprecipitation and its preliminary application for gene regulation.
- Author:
Yong ZHANG
1
;
Xiao-kuan CHENG
;
Ning-hua WU
;
Yu-fei SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Binding Sites; Chromatin; genetics; isolation & purification; Cyclin-Dependent Kinase Inhibitor p21; Cyclins; genetics; metabolism; Gene Expression Regulation; HSP90 Heat-Shock Proteins; genetics; metabolism; Humans; Polymerase Chain Reaction; methods; Precipitin Tests; methods; Promoter Regions, Genetic; Protein Binding; Transcription, Genetic; Tumor Suppressor Protein p53; genetics; metabolism; physiology
- From: Acta Academiae Medicinae Sinicae 2004;26(1):52-55
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo verify the binding of p53 to p21WAF1/CIP1 gene promoter and detect its binding to hsp90 beta gene promoter in vivo.
METHODSChromatin immunoprecipitation and PCR analysis were used to measure specific gene regulation sequence and Western blot analysis to investigate p53 protein.
RESULTSThe p53 binding sequences on the promoters of p21WAF1/CIP1 and hsp90 beta gene were found in the p53 antibody immunoprecipitated DNA fragments and p53 was detected in the immunoprecipitated samples.
CONCLUSIONSp53 binds to promoters of p21WAF1/CIP1 and hsp90 beta gene in vivo, and regulates the expression of the two genes.
