Effects of abnormal structure of sperm chromatin on the outcome of in vitro fertilization and embryo transfer.
- Author:
Long-jie GU
1
;
Zhen-wen CHEN
;
Wen-hong LU
;
Jian-feng XU
;
Mei LI
;
Zi-jiang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Chromatin; genetics; DNA Fragmentation; Embryo Transfer; methods; Female; Fertilization in Vitro; methods; Humans; Infertility; therapy; Male; Pregnancy; Spermatozoa; physiology; Treatment Outcome
- From: Chinese Journal of Medical Genetics 2011;28(2):156-159
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of sperm chromatin structure abnormalities on the outcome of in vitro fertilization and embryo transfer (IVF-ET).
METHODSSperm DNA fragmentation and chromatin packaging defects were assessed in 136 couples undergoing IVF-ET because of infertility. The relationship between sperm DNA fragmentation, chromatin packaging defects and fertilization rate and clinical pregnancy was evaluated.
RESULTSBoth sperm DNA fragmentation and chromatin packaging defect had a negative correlation with fertilization rate (r=-0.198, P<0.05, and r=-0.389, P<0.01, respectively). Both parameters were higher in couples who failed to achieve pregnancy than those who achieved clinical pregnancy (10.74% vs. 5.40%, P<0.01 and 23.58% vs. 11.83%, P<0.01, respectively).
CONCLUSIONAbnormality of sperm chromatin structure is one of the reasons for IVF-ET failure. Examination of sperm chromatin structure is helpful in predicting the risk of IVF-ET failure and optimizing treatment of infertility.
