The RHD zygosity in Dong ethnic population in the southeast area of Guizhou province.
- Author:
Jie HUANG
1
;
Shuo CONG
;
Xiao-jing ZENG
;
Fang YANG
;
Lin-sheng XIAO
Author Information
- Publication Type:Journal Article
- MeSH: China; Ethnic Groups; genetics; Exons; Gene Deletion; Genotype; Heterozygote; Homozygote; Humans; Polymerase Chain Reaction; methods; Rh-Hr Blood-Group System; genetics
- From: Chinese Journal of Medical Genetics 2011;28(2):227-229
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the RHD zygosity distribution and the genetic characteristics of RHD gene in the Dong ethnic population in southeast area of Guizhou province.
METHODSBased on the characteristics of Chinese RHD and the RHD specific deletion, two pairs of primers specific for hybrid Rhesus box and exon 1 of RHD respectively, were designed, combined with a pair of internal control primers. Polymerase chain reaction was performed to genotype the samples.
RESULTSIn the 292 RhD positive samples, 58 (19.86%) were RHD+/RHD- heterozygotes, and the others (80.14%) were RHD+/RHD+ homozygotes. In the 9 D negative samples, 5 were RHD+/RHD- heterozygotes (2 weak D, 3 Del), 3 were RHD+/RHD+ homozygotes (1weak D, 2 Del), and 1 was RHD-/RHD- homozygote.
CONCLUSIONRHD+/RHD- heterozygosity is higher (19.86%) in D positive individuals of Dong ethnic group in Guizhou province than that in other areas; RHD gene heterozygosity is also high in the D negative individuals in this ethnic group.
