Association between single nucleotide polymorphisms of 5'-untranslated region of GPx4 gene and male infertility.
- Author:
Shu-yuan LIU
1
;
Chang-jun ZHANG
;
Xiao-min SI
;
Yu-feng YAO
;
Lei SHI
;
Jin-kun KE
;
Liang YU
;
Li SHI
;
Zhao-qin YANG
;
Xiao-qin HUANG
;
Hao SUN
;
Jia-you CHU
Author Information
- Publication Type:Journal Article
- MeSH: 5' Untranslated Regions; genetics; Adult; Alleles; Gene Frequency; Genotype; Glutathione Peroxidase; genetics; Humans; Infertility, Male; genetics; Linkage Disequilibrium; genetics; Male; Middle Aged; Polymorphism, Single Nucleotide; genetics; Young Adult
- From: Chinese Journal of Medical Genetics 2011;28(3):270-274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the association between the single nucleotide polymorphisms (SNPs) of the 5'-untranslated region (5'-UTR) of phospholipid hydroperoxide glutathione peroxidase (GPx4 or PHGPx) gene and oligo- or asthenozoospermic male infertility.
METHODSThe 5'-UTR region of the GPx4 gene was amplified from infertile men and controls using the polymerase chain reaction and was analyzed for polymorphisms by direct sequencing.
RESULTSA total of 9 SNPs were present in the cohort, however there were no significant differences in these 9 SNPs between the case and control groups. According to the results of linkage disequilibrium analysis and haplotype construction, one haplotype (rs757229-rs757230-rs4588110-rs3746165-rs3746166: C-G-G-T-A) was present only in the control men, and significant difference was detected(P< 0.01).
CONCLUSIONThe SNPs of 5'-UTR region of the GPx4 gene might not be associated with oligo- or asthenozoospermic male infertility. However, the haplotype (rs757229-rs757230-rs4588110- rs3746165-rs3746166: C-G-G-T-A) might be a protective haplotype.
